Apoptotic and Early Innate Immune Responses to PB1-F2 Protein of Influenza A Viruses Belonging to Different Subtypes in Human Lung Epithelial A549 Cells
Identifieur interne : 000222 ( Main/Exploration ); précédent : 000221; suivant : 000223Apoptotic and Early Innate Immune Responses to PB1-F2 Protein of Influenza A Viruses Belonging to Different Subtypes in Human Lung Epithelial A549 Cells
Auteurs : Gunisha Pasricha [Inde] ; Sanjay Mukherjee [Inde] ; Alok K. Chakrabarti [Inde]Source :
- Advances in Virology [ 1687-8639 ] ; 2018.
Abstract
PB1-F2 is a multifunctional protein and contributes to the pathogenicity of influenza A viruses. PB1-F2 is known to have strain and cell specific functions. In this study we have investigated the apoptotic and inflammatory responses of PB1-F2 protein from influenza viruses of diverse pathogenicities in A549 lung epithelial cells. Overexpression of PB1-F2 resulted in apoptosis and heightened inflammatory response in A549 cells. Comparison revealed that the response varied with each subtype. PB1-F2 protein from highly pathogenic H5N1 virus induced least apoptosis but maximum inflammatory response. Results indicated that apoptosis was mediated through death receptor ligands TNF
Url:
DOI: 10.1155/2018/5057184
PubMed: 30687405
PubMed Central: 6330835
Affiliations:
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Chakrabarti</name><affiliation wicri:level="1"><nlm:aff id="I1">Microbial Containment Complex, National Institute of Virology, Sus Road, Pashan, Pune 411021, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Microbial Containment Complex, National Institute of Virology, Sus Road, Pashan, Pune 411021</wicri:regionArea><wicri:noRegion>Pune 411021</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">30687405</idno><idno type="pmc">6330835</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330835</idno><idno type="RBID">PMC:6330835</idno><idno type="doi">10.1155/2018/5057184</idno><date when="2018">2018</date><idno type="wicri:Area/Pmc/Corpus">000895</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000895</idno><idno type="wicri:Area/Pmc/Curation">000895</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000895</idno><idno type="wicri:Area/Pmc/Checkpoint">000170</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000170</idno><idno type="wicri:Area/Ncbi/Merge">000F56</idno><idno type="wicri:Area/Ncbi/Curation">000F56</idno><idno type="wicri:Area/Ncbi/Checkpoint">000F56</idno><idno type="wicri:doubleKey">1687-8639:2018:Pasricha G:apoptotic:and:early</idno><idno type="wicri:Area/Main/Merge">000222</idno><idno type="wicri:Area/Main/Curation">000222</idno><idno type="wicri:Area/Main/Exploration">000222</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Apoptotic and Early Innate Immune Responses to PB1-F2 Protein of Influenza A Viruses Belonging to Different Subtypes in Human Lung Epithelial A549 Cells</title><author><name sortKey="Pasricha, Gunisha" sort="Pasricha, Gunisha" uniqKey="Pasricha G" first="Gunisha" last="Pasricha">Gunisha Pasricha</name><affiliation wicri:level="1"><nlm:aff id="I1">Microbial Containment Complex, National Institute of Virology, Sus Road, Pashan, Pune 411021, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Microbial Containment Complex, National Institute of Virology, Sus Road, Pashan, Pune 411021</wicri:regionArea><wicri:noRegion>Pune 411021</wicri:noRegion></affiliation></author><author><name sortKey="Mukherjee, Sanjay" sort="Mukherjee, Sanjay" uniqKey="Mukherjee S" first="Sanjay" last="Mukherjee">Sanjay Mukherjee</name><affiliation wicri:level="1"><nlm:aff id="I1">Microbial Containment Complex, National Institute of Virology, Sus Road, Pashan, Pune 411021, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Microbial Containment Complex, National Institute of Virology, Sus Road, Pashan, Pune 411021</wicri:regionArea><wicri:noRegion>Pune 411021</wicri:noRegion></affiliation></author><author><name sortKey="Chakrabarti, Alok K" sort="Chakrabarti, Alok K" uniqKey="Chakrabarti A" first="Alok K." last="Chakrabarti">Alok K. Chakrabarti</name><affiliation wicri:level="1"><nlm:aff id="I1">Microbial Containment Complex, National Institute of Virology, Sus Road, Pashan, Pune 411021, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Microbial Containment Complex, National Institute of Virology, Sus Road, Pashan, Pune 411021</wicri:regionArea><wicri:noRegion>Pune 411021</wicri:noRegion></affiliation></author></analytic><series><title level="j">Advances in Virology</title><idno type="ISSN">1687-8639</idno><idno type="eISSN">1687-8647</idno><imprint><date when="2018">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>PB1-F2 is a multifunctional protein and contributes to the pathogenicity of influenza A viruses. PB1-F2 is known to have strain and cell specific functions. In this study we have investigated the apoptotic and inflammatory responses of PB1-F2 protein from influenza viruses of diverse pathogenicities in A549 lung epithelial cells. Overexpression of PB1-F2 resulted in apoptosis and heightened inflammatory response in A549 cells. Comparison revealed that the response varied with each subtype. PB1-F2 protein from highly pathogenic H5N1 virus induced least apoptosis but maximum inflammatory response. Results indicated that apoptosis was mediated through death receptor ligands TNF<italic>α</italic> and TRAIL via Caspase 8 activation. Significant induction of cytokines/chemokines CXCL10, CCL5, CCL2, IFN<italic>α</italic>, and IL-6 was noted in A549 cells transfected with PB1-F2 gene construct of 2008 West Bengal H5N1 virus (H5N1-WB). On the contrary, PB1-F2 construct from 2007 highly pathogenic H5N1 isolate (H5N1-M) with truncated N-terminal region did not evoke as exuberant inflammatory response as the other H5N1-WB with full length PB1-F2, signifying the importance of N-terminal region of PB1-F2. Sequence analysis revealed that PB1-F2 proteins derived from different influenza viruses varied at multiple amino acid positions. The secondary structure prediction showed each of the PB1-F2 proteins had distinct helix-loop-helix structure. Thus, our data substantiate the notion that the contribution of PB1-F2 to influenza pathogenicity is greatly strain specific and involves multiple host factors. This data demonstrates that PB1-F2 protein of influenza A virus, when expressed independently is minimally apoptotic and strongly influences the early host response in A549 cells.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Webster, R G" uniqKey="Webster R">R. G. 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Gilbertson</name></author><author><name sortKey="Mackenzie Kludas, C" uniqKey="Mackenzie Kludas C">C. Mackenzie-Kludas</name></author><author><name sortKey="Barr, I" uniqKey="Barr I">I. Barr</name></author><author><name sortKey="Brown, L" uniqKey="Brown L">L. Brown</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tauber, S" uniqKey="Tauber S">S. Tauber</name></author><author><name sortKey="Ligertwood, Y" uniqKey="Ligertwood Y">Y. Ligertwood</name></author><author><name sortKey="Quigg Nicol, M" uniqKey="Quigg Nicol M">M. Quigg-Nicol</name></author><author><name sortKey="Dutia, B M" uniqKey="Dutia B">B. M. Dutia</name></author><author><name sortKey="Elliott, R M" uniqKey="Elliott R">R. M. Elliott</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Pasricha, Gunisha" sort="Pasricha, Gunisha" uniqKey="Pasricha G" first="Gunisha" last="Pasricha">Gunisha Pasricha</name></noRegion><name sortKey="Chakrabarti, Alok K" sort="Chakrabarti, Alok K" uniqKey="Chakrabarti A" first="Alok K." last="Chakrabarti">Alok K. Chakrabarti</name><name sortKey="Mukherjee, Sanjay" sort="Mukherjee, Sanjay" uniqKey="Mukherjee S" first="Sanjay" last="Mukherjee">Sanjay Mukherjee</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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